{"id":1326,"date":"2020-08-04T09:06:54","date_gmt":"2020-08-04T02:06:54","guid":{"rendered":"http:\/\/sci.edu.vn\/?p=1326"},"modified":"2024-11-24T07:49:42","modified_gmt":"2024-11-24T00:49:42","slug":"mnc-extraction-easy-kit","status":"publish","type":"post","link":"https:\/\/sci.edu.vn\/home\/blog\/2020\/08\/04\/mnc-extraction-easy-kit\/","title":{"rendered":"MNC Extraction Easy Kit"},"content":{"rendered":"\n

Cat. No:<\/strong> K009<\/strong><\/span><\/p>\n\n\n\n

Th\u00f4ng tin chung<\/span><\/strong><\/p>\n\n\n\n

\"\"<\/a><\/figure><\/div>\n\n\n\n

            MNC Extraction Esay Kit l\u00e0 s\u1ea3n ph\u1ea9m ch\u1ee9a h\u00f3a ch\u1ea5t v\u00e0 d\u1ee5ng c\u1ee5 \u0111\u1ed3ng b\u1ed9 \u0111\u1ec3 t\u00e1ch t\u1ebf b\u00e0o c\u00f3 nh\u00e2n <\/span><\/em><\/strong>(mononuclar cells – MNC)<\/em> t\u1eeb m\u00e1u d\u00e2y\/cu\u1ed1ng r\u1ed1n, t\u1ee7y x\u01b0\u01a1ng hay m\u00e1u ngo\u1ea1i vi. Kit t\u00e1ch t\u1ebf b\u00e0o MNC d\u1ef1a tr\u00ean nguy\u00ean t\u1eafc li t\u00e2m \u0111\u1eb3ng t\u1ec9 tr\u1ecdng v\u1edbi dung d\u1ecbch c\u00f3 t\u1ec9 tr\u1ecdng 1,077 g\/mL. \u0110\u1ec3 d\u1ec5 d\u00e0ng thao t\u00e1c, kit c\u00f3 s\u1eed d\u1ee5ng 1 l\u1edbp gel b\u00e1n r\u1eafn \u0111\u1ec3 ng\u0103n c\u00e1ch t\u1ebf b\u00e0o MNC v\u00e0 c\u00e1c t\u1ebf b\u00e0o c\u00f2n l\u1ea1i sau khi t\u00e1ch. L\u1edbp gel n\u00e0y c\u00f3 t\u1ec9 tr\u1ecdng nh\u1ecf h\u01a1n t\u1ec9 tr\u1ecdng t\u1ebf b\u00e0o MNC nh\u01b0ng l\u1edbn h\u01a1n c\u00e1c t\u1ebf b\u00e0o b\u1ea1ch c\u1ea7u c\u00f2n l\u1ea1i v\u00e0 h\u1ed3ng c\u1ea7u.<\/p>\n\n\n\n

Th\u00e0nh ph\u1ea7n<\/span><\/strong><\/p>\n\n\n\n

            S\u1ea3n ph\u1ea9m g\u1ed3m:<\/strong><\/p>\n\n\n\n

  • 2 \u1ed1ng MNC Extraction kit 15 mL (c\u00f3 th\u1ec3 ch\u1ee9a t\u1ed1i \u0111a 10 mL m\u00e1u \u0111\u00e3 ch\u1ed1ng \u0111\u00f4ng)<\/li>
  • 1 \u1ed1ng ch\u1ee9a dung d\u1ecbch Washing Buffer 50 mL<\/li>
  • 1 kim d\u00e0i v\u00f4 tr\u00f9ng<\/li>
  • 1 syringe 10mL v\u00f4 tr\u00f9ng<\/li><\/ul>\n\n\n\n

    Quy tr\u00ecnh s\u1eed d\u1ee5ng<\/span><\/strong><\/p>\n\n\n\n

    • Thu m\u00e1u cu\u1ed1ng r\u1ed1n hay m\u00e1u ngo\u1ea1i vi, ho\u1eb7c t\u1ee7y x\u01b0\u01a1ng \u0111\u01b0\u1ee3c thu nh\u1eadn v\u00e0 ch\u1ed1ng \u0111\u00f4ng trong dung d\u1ecbch th\u00edch h\u1ee3p<\/li>
    • H\u00fat t\u1ed1i \u0111a 10 mL m\u00e1u \u0111\u00e3 ch\u1ed1ng \u0111\u00f4ng cho v\u00e0o \u1ed1ng MNC Extraction, \u0111\u1eady k\u00edn n\u1eafp<\/li>
    • Ly t\u00e2m 1800 G trong 20 ph\u00fat \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, brake = 0.<\/li>
    • Thu MNC t\u1eeb l\u1edbp d\u1ecbch n\u1eb1m ngay tr\u00ean l\u1edbp gel b\u00e1n r\u1eafn b\u1eb1ng c\u00e1ch \u0111\u01b0a kim d\u00e0i n\u1ed1i v\u1edbi syringe 10 mL h\u00fat t\u1eeb l\u1edbp d\u1ecbch tr\u00ean l\u1edbp gel \u0111\u1ebfn h\u1ebft; cho v\u00e0o \u1ed1ng li t\u00e2m 15 mL v\u00f4 tr\u00f9ng kh\u00e1c<\/li>
    • H\u00fat, b\u1ed5 sung dung d\u1ecbch Washing Buffer v\u00e0o \u1ed1ng li t\u00e2m ch\u1ee9a ph\u00e2n \u0111o\u1ea1n MNC \u0111\u1ec3 \u0111\u1ea1t 12-15 mL trong \u1ed1ng li t\u00e2m 15 mL m\u1edbi<\/li>
    • Li t\u00e2m t\u1ea1i 1500 g trong 5 ph\u00fat v\u00e0 thu c\u1eb7n<\/li>
    • R\u1eeda c\u1eb7n b\u1eb1ng dung d\u1ecbch Washing Buffer 2 l\u1ea7n<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"

      Cat. No: K009 Th\u00f4ng tin chung             MNC Extraction Esay Kit l\u00e0 s\u1ea3n ph\u1ea9m ch\u1ee9a h\u00f3a ch\u1ea5t v\u00e0 d\u1ee5ng c\u1ee5 \u0111\u1ed3ng b\u1ed9 \u0111\u1ec3 t\u00e1ch t\u1ebf b\u00e0o c\u00f3 nh\u00e2n (mononuclar cells – MNC) t\u1eeb m\u00e1u d\u00e2y\/cu\u1ed1ng r\u1ed1n, t\u1ee7y x\u01b0\u01a1ng hay m\u00e1u ngo\u1ea1i vi. Kit t\u00e1ch t\u1ebf b\u00e0o MNC d\u1ef1a tr\u00ean nguy\u00ean t\u1eafc li t\u00e2m \u0111\u1eb3ng t\u1ec9 […]<\/p>\n","protected":false},"author":291,"featured_media":1329,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[160],"tags":[69,68],"class_list":["post-1326","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cong-nghe","tag-kit","tag-mnc"],"jetpack_featured_media_url":"https:\/\/sci.edu.vn\/home\/wp-content\/uploads\/2020\/08\/MNC-extraction-ez-kit-e1596708141418.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/posts\/1326","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/users\/291"}],"replies":[{"embeddable":true,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/comments?post=1326"}],"version-history":[{"count":3,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/posts\/1326\/revisions"}],"predecessor-version":[{"id":1353,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/posts\/1326\/revisions\/1353"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/media\/1329"}],"wp:attachment":[{"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/media?parent=1326"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/categories?post=1326"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sci.edu.vn\/home\/wp-json\/wp\/v2\/tags?post=1326"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}